chapter  4
30 Pages

A Low-Budget Luminometer for Sensitive Chemiluminescent Immunoassays*

The enzyme-linked immunosorbent assay (ELISA) for antigens and antibodies with one of the reagents bound to a solid phase is in widespread use. The final readout of the enzyme product is most commonly made spectrophotometrically using a chromogenic substrate in 96-well microtiter plates, but there is now a general movement toward the development of chemiluminescent rather than colorimetric probes because of low backgrounds, large linear concentration ranges, and sensitivities that may be 10-to 100-fold greater.