ABSTRACT
The study of internalization kinetics of cell-penetrating peptides (CPPs) is an important tool for understanding the uptake process. Not only does it reveal the rate by which CPPs are taken up by cells, but it can also give additional insights, particularly in combination with other methods. For instance, the quantity of a CPP maximally
accepted by the cell can be predicted. Studies of uptake kinetics are essential in order to achieve a better understanding of the structure-activity relationship of CPPs. Furthermore, data on the efficiency and limitations of CPPs for the transfer of different cargoes in different cells could be amassed. Finally, studies of kinetics could provide a very important contribution for elucidation of the mechanisms by which CPPs pass membranes and enter cells.
