ABSTRACT

Glossary of Terms .......................................................... 376 References ........................................................................ 376

The advent of molecular-based diagnostics has resulted in rapid advancements in investigation and diagnosis of infectious diseases in reptiles. Use of molecular techniques has resulted in a rapid proliferation of the number of reptile pathogens that can be identified, and a dramatic increase in the sensitivity and specificity of testing for them. As with any technique, the reliability of molecular diagnostics depends on the care and skill of the operator. In order to properly evaluate molecular diagnostic testing, it is crucial to understand the fundamentals of the various tests and assays. This chapter is designed to familiarize veterinarians with the basics of the molecular-based diagnostics currently employed in reptile medicine. The nuts and bolts of the most common diagnostic tests will be discussed, including their advantages and disadvantages, as well as pitfalls to consider when evaluating test results. We hope the information provided will aid clinicians and pathologists in the evaluation and interpretation of molecular-based diagnostic tests.