chapter  1
20 Pages

The Physiological Role of Carbon Monoxide Derived from Heme Oxidation Catalyzed by Heme Oxygenase

I. Introduction ......................................................................................................4 A. Glyceryl Trinitrate and Nitric Oxide ....................................................4 B. Studies of Glyceryl Trinitrate Biotransformation

Using Carbon Monoxide.......................................................................4 C. Formulation of Hypothesis — Does Carbon Monoxide Have a

Physiological Function? ........................................................................5 II. Experimental Investigations Directed to Determine Whether

Carbon Monoxide Has a Physiological Role................................................6 A. Presence of Heme Oxygenase in Blood Vessels ..................................6 B. Endogenous Formation of Carbon Monoxide

in Blood Vessels and Other Tissues......................................................7 C. Use of Metalloporphyrin Inhibitors to Study

Possible Physiological Role of Carbon Monoxide...............................7 D. Effect of Inhibition of Soluble Guanylyl Cyclase

on Vascular Effects of Carbon Monoxide...........................................10 E. Potentiation of Carbon Monoxide-Induced Relaxation

of Rat Aorta by YC-1 [3-(5

-Hydroxymethyl-2

-Furyl)- 1-Benzylindazole]................................................................................11