chapter  21
30 Pages

Plant-Associated Toxins: Bioactivity-Guided Isolation, ELISA, and LC-MS Detection

WithSteven M. Colegate

Glucosides from Stemodia kingii ........................................................................................ 556 21.2.1 Clinical and Pathological Descriptions of the Intoxication of Sheep .................... 556 21.2.2 Development and Validation of a Mouse Bioassay ............................................... 557 21.2.3 Isolation of the Toxins ........................................................................................... 557

21.2.3.1 Extraction .............................................................................................. 557 21.2.3.2 Chromatography ................................................................................... 557

21.2.4 Structural Elucidation ............................................................................................ 558 21.2.4.1 Physicochemical Analysis ..................................................................... 558 21.2.4.2 High Pressure Liquid Chromatography-Mass Spectrometry .............. 558 21.2.4.3 NMR Spectroscopy .............................................................................. 559

21.2.5 Conclusions and Future Research ......................................................................... 561 21.3 The In Vitro Detection of Hepatocytotoxic Fungal Metabolites Potentially

Related to Acute Bovine Liver Disease .............................................................................. 563 21.3.1 Description of the Intoxication .............................................................................. 563 21.3.2 Detection of Hepatocytotoxins ..............................................................................564