ABSTRACT
The G protein βγ complex is now known to play important roles in specifying receptor contact and in modulating the activity of a wide variety of effectors.1,2 The ability to interact with other proteins is thus central to the function of the βγ complex. To determine the basis of these interactions different techniques have been used. We focus here on assays that we have used to understand βγ subunit function. These assays focus on three different important interfaces — (i) subunit-subunit; (ii) subunit-effector; and (iii) receptor-subunit.
