ABSTRACT
We have reported previously that the level of lipid hydroperoxide in the brain tissue was significantly higher in SAMP8 than in SAMR1. To investigate the implication of oxidative stress in the development of learning and memory deficits observed in senescence-accelerated mouse (SAMP8), we analyzed the lipid hydroperoxide components and the amount of each in various brain parts (the olfactory center, septum, hippocampus, cortex, cerebellum, pons, and other parts including thalamus) using the HPLC-luminol chemiluminescence method. The levels of phosphatidylcholine hydroperoxide (PCOOH), phosphatidylethanolamine hydroperoxide (PEOOH), and phosphatidylserine hydroperoxide (PSOOH)
in the hippocampus of SAMP8 were significantly higher than in those of SAMR1. In addition, the PCOOH level in the cortex was significantly higher in SAMP8 than in SAMR1. These results suggested that the severe oxidative stress observed in SAMP8 might be related to their learning and memory deficits.
