chapter  5
Flow Cytometry
ByFrancis Mandy, Joe Trotter, Rudi Varro, Ming Yan, Diether Recktenwald
Pages 28

Flow cytometry allows the analysis and sorting of particles of biological interest at rates of more than 104 s−1, based on the analysis of light scatter and uorescence. It also permits the quantitative analysis of many cellular constituents based on uorescence measurements. Measurements at the single-molecule level have been reported. Based on the versatility and richness of information of ow cytometry, it is used in biological and biomedical research and for clinical data collection. is chapter provides an overview of ow cytometry and its biomedical and clinical applications. For readers interested in further details,

5.1 Introduction ......................................................................................141 5.2 Hardware ............................................................................................142 5.3 Fluidics ...............................................................................................142 5.4 Optics ..................................................................................................143 5.5 Electronics .........................................................................................144 5.6 Cell Sorting with Cloning ...............................................................145 5.7 Data Analysis .....................................................................................145 5.8 Flow Cytometry Measurements .....................................................146 5.9 Flow Cytometer Characterization and Setup ...............................146 5.10 New Approaches to Data Transformation and Analysis ............147