chapter  6
23 Pages

siRNA targeting using injectable nano-based delivery systems

ByLan Feng, Russell J. Mumper

OVERVIEW RNAi Mechanisms and siRNA Antisense is a ubiquitous and conserved phenomenon in cells. Antisense nucleotides suppress the gene expression through several distinct mechanisms, such as RNaseH-induced degradation of complimentary mRNA through antisense oligonucleotides hybridizing to their target mRNA; sterical inhibition of mRNA translation or pretranslational splicing; cleavage of target mRNA by some ribozymes or deoxyribozymes because of their intrinsic catalytic activity; RNA-induced silencing complex (RISC)-mediated degradation of target mRNA by doublestranded RNA (dsRNA) (1-3).