chapter  32
20 Pages

Nitrosamines

BySUSANNE RATH AND FELIX GUILLERMO REYES REYES

Contents 32.1 Introduction ................................................................................................................. 687 32.2 Chemistry .................................................................................................................... 688 32.3 Formation and Occurrence in Meat and Meat Products .............................................. 690 32.4 Toxicological Aspects ....................................................................................................691 32.5 Regulatory Aspects ........................................................................................................691 32.6 Analytical Aspects ........................................................................................................ 692

32.6.1 Sample Preparation ........................................................................................ 696 32.6.1.1 Distillation and Clean-Up Procedures ........................................... 696 32.6.1.2 Solvent Extraction Followed by Cleanup Using Liquid-Solid

Extraction ...................................................................................... 696 32.6.1.3 Matrix Solid-Phase Dispersion and Liquid-Liquid Extraction....... 697 32.6.1.4 Solid-Phase Microextraction .......................................................... 698 32.6.1.5 Supercritical Fluid Extraction ........................................................ 699 32.6.1.6 Quantitation Methods ................................................................... 699

32.7 Conclusions...................................................................................................................702 References ................................................................................................................................702

32.1 Introduction Nitrosamines are N-nitroso compounds that have received considerable attention worldwide during the past half century, since Barnes and Magee1 fi rst reported in 1954 the association between dimethylnitrosamine (NDMA) and liver damage in rats. Two years later, the same British scientists confi rmed the induction of liver tumors in rats by feeding them NDMA.2