chapter  6
8 Pages

Fertilization: Pronuclear Formation and Fading

ByLouise Kellam, Laina Murphy

Observation of the presence and number of pronuclei is regarded as a reliable method of confirming fertilization. Observing the dynamic process of pronuclear formation and fading, however, has not been possible without time-lapse technology. Video 6.1 shows an oocyte following ICSI showing second polar-body extrusion, pronuclear formation, and fading. The video can be viewed online: