chapter  1
6 Pages

Equipment Required for Time Lapse Observations in In Vitro Fertilization

ByLouise Best

Time-lapse imaging of human embryos has been used since the 1990s to study fertilization and early human embryo kinetics.1

This early time-lapse cinematography collected images of inseminated oocytes every minute, for a period of 4 hours, using a low-light colour video camera positioned within a Perspex temporary incubation chamber on an inverted microscope using ×200 magnification which was augmented to ×1064 on a monitor. Technology has advanced since then to allow successive, noninvasive, interval imaging of an oocyte throughout the duration of the culture period from insemination onward in a culture dish without the need for it to be removed from its stable culture environment, until embryo transfer or cryopreservation.