ABSTRACT
All of these amines are highly polar, water-soluble substances. Their advantages and disadvantages must be determined empirically for each biological reaction system. For best buffering performance they should be used at pH's close to the pKa, preferably within ± 0.5 pH units of the pKa and never more than ± 1.0 unit from the pKa. Note that the pKa's, and therefore the pH's of buffered solutions, change with temperature in the manner indicated.
