ABSTRACT
Chromatographic stationary phases are offered in a staggering array of chemical diversity. One of the key means of organizing stationary phases has been on the basis of their functional groups that enable classification into broad classes of operating modes. While much has been written about each of these operational modes in the literature, this chapter aims at providing a readily accessible, single source of basic information that a new practitioner in bioseparations would find useful. The chapter provides concise information about the interaction mechanism in each mode of chromatography and practical considerations that are important to bear in mind on each of them. Not included are topics such as mass transport and kinetics considerations, the stationary phase morphology or large-scale column packing and operation. Since these considerations are not necessarily specific to the mode of interaction, they are not considered here. The classification of chromatographic modes used in this chapter is shown in Figure 6.1.
