chapter  9
30 Pages

Lifetime-Based Imaging

Time-resolved fluorescence is of interest because the molecular information content is greater than for steady-state measurements. However, the information content of time-resolved fluorescence is usually not accessible with imaging techniques such as fluorescence microscopy. The situation has changed during the last decade as remarkable developments in time-resolved fluorescence facilitated the transfer of time-resolved fluorescence from solution spectroscopy to the field of lifetime-based sensing and imaging.