chapter  55
72 Pages

Amplification Techniques for Optical Detection

This chapter presents a summary account of various amplification techniques for analysis of biological molecules (species) that use some form of optical detection as the endpoint. The analyte species possibly under consideration include lipids, carbohydrates, proteins, and nucleic acids (and combinations of the same such as glycolipids, glycoproteins, etc.), but this review is restricted to proteins and nucleic acids. Of course, there are many detection strategies for both proteins and nucleic acids, but for the purposes of this review, amplification must be involved. We therefore have limited our discussion to immunoassays and nucleic acid techniques employing amplification.*

Immunoassays, which depend fundamentally on antibody recognition of antigens, have proved, over the course of many years, to be powerful analytical techniques for protein detection. They can also be used successfully for the detection of substances of lower molecular weight than the usual size of proteins (e.g., peptides, hormones, etc.), provided that antibodies specific against these molecular structures are developed and specially selected.