ABSTRACT
For small and medium-scale genotyping, inexpensive PCR-RFLP (Restriction Fragment Length Polymorphism) (Chang, Cheng, Chuang, & Yang, 2010; Chang, Yang, Chang, Cheng, & Chuang, 2006; Chuang et al., 2008) is preferable. However, PCR-RFLP takes long digestion period (usually 2-3 hours) of the restriction enzymes to limit its efficiency.
