ABSTRACT
The injection of calcium-binding fluorescent dyes is a well-established method to label the different stages of bone remodelling.The chelating fluorochromes and their dosages as well as their recommended sequence for in vivo administration were described by Rahn (1977). The methodology used to assess bone remodelling by fluorochrome labelling differs if the observation is carried out by fluorescence (Lee et al, 2003; O’Brien et al, 2007; Pautke et al, 2005, 2010; Lee et al, 2010; Cacchioli et al, 2011) or by confocal microscopy (Jones et al, 2005; Maggiano et al, 2006; Smith et al, 2006; Pilloli et al, 2008, Manresa et al, 2013). However, as reported by Van Gaalen et al (2010), despite the fact that this labelling technique has been used for years in bone tissue studies, it lacks a standardized protocol. Moreover no studies have been carried out to compare both observation methods.
