ABSTRACT
Selenium (Se) is an essential micronutrient for mammals and certain algae, although the difference between deficient, essential, and toxic doses of Se is small. The in vitro simulated gastrointestinal digestion test, which has the advantages of low cost, simple operation, and easy control of conditions, is a good approach to determine the element bioaccessibility under laboratory conditions and evaluate the health risk of Se-enriched products. Lentinula edodes was selected as the material of Se biofortification. Exogenous Se supplements were injected into cultivation substrate to produce Se-enriched L. edodes fruit bodies. The highest utilization of Se was observed with the selenite treatment. The Se bioaccessibility was high in Se-enriched fruit bodies harvested from each Se treatment. In view of the double effects of Se on health, both total Se content and the food safety of the Se bio-fortified products need to be evaluated.
