ABSTRACT
This chapter explains the analysis of function of selenoproteins by bioinformatics techniques in a genome-wide perspective to identify the core selenoproteins, which can be used in re-recognizing the role of selenoproteins in complex diseases. The mRNA levels of core members of selenoproteins in Kashin-Beck Disease (KBD) patients were test by RT-qPCR. Through the above analyses, the authors aim to identify the core selenoproteins, their main biological functions, and their key signaling pathways. Furthermore, the mRNA levels of core selenoproteins were detected in KBD patients to provide an experimental basis for exploring the pathogenic mechanism of KBD. RT-qPCR was used to test the mRNA levels of the core selenoproteins both in KBD and controls according the PPI network. RT-qPCR results showed that mRNA levels of SELENOS, TXNRD1, TXNRD2, and TXNRD3 in peripheral blood mononuclear cells of KBD patients were decreased compared with controls.
