ABSTRACT
I. Introduction 260
II. Imaging Methodology 260
A. Bioluminescent Reaction 260
B. Tissue Optics 261
C. Imaging System Design and Function 265
III. Applications 266
A. Transcription Factors and the Lungs 266
B. Detection of NF-kB Activation in Reporter Mice 267 C. NF-kB Activation in Disease Models 271
Lung Inflammation Induced by Bacterial LPS 271
Lung Inflammation Following Direct Hepatic Injury
and Pancreatitis 273
Pseudomonas Pneumonia 274
Gene Therapy to Modulate NF-kB Activation 274 D. Summary 276
IV. Challenges and Future Directions 276
References 279
*Supported by NIH grants HL61419, HL66196, P20 CA86283 and the Whitaker
Foundation.
