ABSTRACT
Phage library technology is a powerful tool for targeting antibodies (Abs) of known or unknown specificity. In this chapter, we examine techniques for applying this technology to Ab-dependent applications, specifically, epitope mapping and the development of diagnostics and vaccines. Abs produced during bacterial and viral infections, allergic responses or chronic illnesses, such as autoimmune disease and cancer, can provide information about the immune response, and
sometimes, disease etiology. A proportion of serum Abs may be biologically active. For example, some may neutralize virus or block bacterial infection, whereas others may trigger cell lysis by complement or mediate Ab-dependent cellular cytotoxicity (ADCC). Phage display libraries can be screened with Abs to map epitopes on protein antigens (Agns) and to study ligand interactions. Moreover, ligands for biologically active Abs can serve as candidate leads for therapeutic and vaccine development.
