ABSTRACT

Cytotoxic testing is needed as an initial screening to assess the effect of a natural substance inhibiting tumor cell growth in the development of new drugs for cancer therapy agents. A flavonoid derivatives extracted from gynura divaricata is one of the compounds being investigated as an anticancer. The aim of this study was to assess the cytotoxic activity of gynura divaricata leaf in the culture of carcinoma mammae cells and to compare them with standard drugs. Cytotoxic activity of gynura divaricata leaf water extract and doxorubicin was investigated using the MTT method on T47D cell line culture. The absorbance of each well was measured at a wave length 595 nm using a spectrophotometer. Using Probhit analysis, the absorbance results were calculated to create a cytotoxic curve. The result showed inhibition concentration (IC50) value 102.32 μg/mL for gynura divaricata water exact and 10,30 μg/mL for doxorubicin. This result indicated that gynura divaricata leaf water extract could inhibit 50% tumor cell growth at concentration 102. 32 μg/mL. It was concluded that gynura divaricata leaf has potential anti-cancer activity (IC50<200 μg/mL) against carcinoma mammae cell, but not as strong as doxorubicin.