ABSTRACT
Over-expression of miRNA-16 inhibits proliferation, invasion, and metastasis of hepatocellular carcinoma (HCC). Detection of miRNA levels has potency for the initial diagnosis of malignancy, prognosis prediction and can monitor the therapeutic response because miRNA is very stable in the blood. It was hypothesized that flavonoid from clove leaf oil will be able to interfere MiRNA-16 expression in liver cancer cells. The aim of the study is to analyse miRNA-16 expression on liver cancer cell culture after administration of pure compound from clove leaf oil. This study used the HepG2 cell line culture and treatment groups were given pure compound isolated from clove leaf oil. Measurement of gene expression was done with real-time PCR. In this study, the measurement of miRNA-16 expression in liver cancer cell culture after administration of flavonoid pure compound from clove leaf oil showed an increase in expression more than five times the level of expression of the control group. This means that flavonoid compound from clove leaf oil can induce miRNA-16 expression on liver cancer cells culture. This result provides a preclinical proof-of-concept for a potential strategy for HCC treatment based on the regulation of miRNA-16 expression with clove leaf as novel therapy agent.
