ABSTRACT
This chapter deals with a method of determining the elution profile of a solute of which the equilibrium isotherm follows a type II isotherm. Large-scale preparative liquid chromatography is an important separation technique for isolation and purification of biologically produced chemicals. A reasonable starting point in any attempt to develop a relationship between effluent concentration profile and operating variables is the theory of the ideal equilibrium process. It serves as a useful approximation to real behavior. The theory of ideal equilibrium fixed-bed adsorber serves as a useful approximation to real behavior. The methods of characteristics have been used efficiently for the breakthrough analysis. The method developed is valuable in understanding liquid chromatographic operations and in making rapid calculations for the design, scale-up, and optimization of liquid chromatography. Desorption of the solute is usually initiated when the solute concentration in the effluent stream reaches or passes the breakthrough value, in other words, before the bed is completely saturated.
