ABSTRACT
This chapter shows that immunological techniques are being used successfully in various areas of current plant research. The benefit and applicability of monoclonal antibody technology in plant bioregulation research will be made apparent. The chapter presents technical and procedural details so that investigators can produce monoclonal antibodies for use in studying plant bioregulation. Auxin is one of the earliest known and best studied endogenous growth regulators, has an important role in cell division, growth, and differentiation, and is one of the earliest plant molecules used to produce antibodies within rabbit sera. Growth regulation is a complicated and well-coordinated phenomenon controlled by a number of regulatory systems at the level of a molecule, cell gene-chromosome, tissue, and organism. Plant growth regulators have been used successfully in many ways to improve agricultural production.
