ABSTRACT
Flow cytometry has increasingly found important applications in the study of higher plants, their cells, and organelles. Plants have provided some of the most intriguing challenges to the routine application of this technology. This is partly because higher plants are not suspensions of single cells, but typically comprise complex three-dimensional tissues. For plant tissues to be studied through flow cytometry, suspensions of single cells or organelles must be prepared before analysis. The act of preparing plant cells for flow cytometry involves perturbation of their normal environment, and this can alter the parameters measured through flow cytometry.
