ABSTRACT
C-Reactive Protein is present in the blood of normal individuals in trace amounts, but its concentration rises to more than 300 mg/l soon after the onset of inflammation, and then returns to a low basal level after the inflammatory stimulus is removed. The study of acute phase response has attracted substantial interest not only for its medical implications, but also because it provides an excellent system with which to elucidate the molecular mechanisms involved in the modulation of gene expression. Hemopexin is a heme-binding plasma glycoprotein composed of a single 439-amino acid-long polypeptide chain. The proteins were then transferred onto nitrocellulose and probed with a 1:100 dilution of anti-tag antibody. Antigenantibody complexes were then detected by use of alkaline phosphatase-coupled goat antimice antibodies. Immunofluorescence staining was performed on cells grown on coverslips and transfected with 2.5 μg of recombinant plasmid.
