ABSTRACT
This chapter reviews the recent advances in our understanding of the assembly and sorting of proteins within the Endoplasmic Reticulum and presents data which indicate that the intracellular trafficking of newly synthesized C-reactive protein is governed by a novel post-translational mechanism which is regulated during the acute phase response. Homogenization-induced leakage of pulse-labeled proteins and adsorption of leaked proteins to microsomal fractions were estimated employing a strategy described previously. The efficacy of the fractionation procedure was assessed by determination of specific marker enzyme activities. The kinetics of C-reactive protein and albumin secretion were studied in cultures prepared from animals manifesting varying degrees of the acute phase response to in vivo inflammatory stimulus.
