ABSTRACT
The amplification products are separated on a standard sequencing gel and visualized via autoradiography. Variation in Polymerase Chain Reaction (PCR) product length is a function of the number of Simple Sequence Repeat (SSR) units. Botstein et al. heralded the era of DNA based molecular genetic markers by proposing that Restriction Fragment Length Polymorphisms provide the basis of a new type of genetic linkage map. Based upon sequence data obtained from the soybean clones described, numerous SSR loci have been identified in that produce single PCR products in homozygous soybean genotypes. Because of the abundance and co-dominant inheritance these markers provide an excellent tool for genetic mapping. The accumulating data suggest that SSR loci are quite abundant in a number of plant species. SSR markers may be particularly useful in important species such as tomato and hexaploid wheat in which molecular genetic diversity is particularly low among cultivated varieties.
