ABSTRACT
This book presents a summary of the application and instrumentation of cell electrophoresis. The method of making cell purification and characterization possible according to the cellular negative surface charge density is discussed, and ideas for future developments are explained. The negative electrostatic forces at cell surfaces provide information about cell-cell interaction, blood vessel sealing, cytokine actions, cell transformation, ion transport phenomena, and other biological phenomena. Recalculations of the physical principles of cell electrophoresis reveal possibilities for removing disruptive factors caused by electrical current, heat, and sedimentation. The introduction of computer technology, the performance of simultaneous two-parameter measurements, and the application of cell-friendly but current-inert buffer systems render the method more reliable and efficient.
TABLE OF CONTENTS
part I|53 pages
Theoretical Considerations
chapter Chapter 2|21 pages
Numerical Description of Zone Electrophoresis in the Continuous Flow Electrophoresis Device
part II|88 pages
Improvements of the Method
part III|54 pages
Buffers and Their Effects on Cells
chapter Chapter 8|17 pages
Alteration of Cellular Features after Exposure to Low Ionic Strength Medium
part IV|84 pages
The Importance of Determination of the Negative Surface Charge Density of Cells
part V|39 pages
Cell Electrophoresis in Space