Role of the acrosome in the fertility pathway

At first, the human sperm acrosome was deemed too small to be visualized by direct microscopy, and electron and transmission electron microscopy was used or advocated. However, the ability to evaluate acrosome morphology or status by light microscopy is now acknowledged13. With good bright-field optics, at 1000× or preferably 1250× magnification and good Papanicolaou staining of the spermatozoa, valuable information on acrosomal morphology can be obtained1,11.