ABSTRACT
Ethyl acetate fraction of Andrographis paniculata ethanol extract (namely AS202–01) has been proved as an active antimalarial agent in Plasmodium berghei-infected mice. The aim of this study was to determine the antimalarial activity of AS202–01 tablets in P. berghei-infected mice and survival time in mice models. The antimalarial suppressive test was conducted using Peter’s test for 4 days. P. berghei-infected mice were treated with AS202–01 tablets at doses of 6.25, 12.5, 25, and 50 mg andrographolide/kg. Acute toxicity test was conducted in mice treated with tablets at doses of 5, 50, 300, and 2,000 mg/kg and observed every 4 h for 14 days. The results indicated that AS202–01 tablets had an ED50 value of 9.195 mg/kg and the mean survival time of >12 days. An LD50 value of >2,000 mg/kg was found with no histopathological changes in the liver and kidneys. In conclusion, the AS202–01 tablet exhibited antimalarial activity and was classified as a relatively nontoxic substance.
