chapter  8
23 Pages

Inverse Size-Exclusion Chromatography

ByAnnamária Sepsey, Ivett Bacskay, Attila Felinger

This chapter explains the most important theories and the newest applications regarding inverse size-exclusion chromatography (ISEC). ISEC is a very comfortable and preferred method to derive information about the structure of the porous packing material, such as the interstitial porosity and the surface area of the packed bed or pore size distribution of various porous particles and monoliths. ISEC has several advantages in comparison to other classical or novel methods for determining the porous structure. In addition, ISEC is operated under typical chromatographic conditions, so it is a relatively convenient approach. ISEC is a powerful tool to determine the size and the distribution of the stationary phase pores in liquid chromatography. The stochastic theory seems rather suitable for describing size exclusion chromatography among the basic theories of chromatography because it describes the chromatographic process at the molecular level.