ABSTRACT
This chapter discusses the advances in the development of nanobiocatalytic systems through the immobilization of different enzymes onto various graphene- based nanomaterials (GBNs). The development of effective nanobiocatalytic systems mainly depends on the use of suitable nanosupports for enzyme immobilization. An interesting immobilization technique is based on the modification of carbon-based nanomaterials including graphene and carbon nanotubes by 1-pyrenebutanoic acid succinimidyl ester. The surface chemistry of the nanomaterial seems to play a critical role not only in the conformational state but also in the biocatalytic activity of immobilized enzymes. GBNs have been also used for the immobilization of different oxidoreductases for the degradation of pollutants of environmental concern, such as phenolic compound and chlorinated aromatic compounds. Graphene-based nanomaterials were employed for the development of immobilized enzyme systems that could be promising for wastewater treatment, as well as for the conversion of biomass to biofuels.
