ABSTRACT
Propagation through plant tissue culture has gained importance as it is rapid with no seasonal constrains and plants can be multiplied throughout the year. These in vitro plants are derived through asexual mode of regeneration and may develop as somaclonal variants. The reason may be due to preexisting variation in the explants or are generated during culture. These somaclones can be detected by different techniques like morphological, physiological, biochemical, protein and isozyme or molecular markers. Somaclonal variations in form of off-types are undesirable in micropropagation program, as the main goal is to generate true-to-type progeny. But on the other hand it provides genetic variability which has applications in plant breeding as well as in genetic improvement of the plant species.
