chapter  37
21 Pages

High-Throughput Screening of Extracellular Matrix–Based Biomaterials

WithCintia D. S. Horinouchi, Willeke F. Daamen, Ruud A. Bank, Toin H. van Kuppevelt

In this chapter, natural materials will be discussed with focus on materiomics and high-throughput means of analysis. First, a tutorial is given on the extracellular matrix, a set of molecules which forms the cellular microenvironment in the tissues, since these components are often used as the basis for biomaterials in tissue engineering and regenerative medicine. Next, arrays of biological materials are described, in which multiple biological materials can be simultaneously tested through of in vitro assays to select suitable molecules to produce scaffolds for in vivo usage. Finally, a gene 1092expression–based method to analyze the in vivo performance of (natural) biomaterials is described using DNA microarrays and gene ontology (GO) analysis.