ABSTRACT
An enzymatic biosensor is known to utilize an enzyme as a recognition element and considered as an established and reliable tool for clinical diagnostics. It is an integration of the biocatalyst (catalytic reaction with a target analyte) and transducer material (transduced signal via optically or electrically). The enzymatic biosensors provide easy, simple, cost-effective, and fast detection of various target analytes including glucose, cholesterol, urea, and so on in serum, urine, body fluids, and so on. A large number of enzymebased biosensors has been developed using nanostructured materials. This chapter deals with the basic principle of enzymatic biosensor, techniques for enzyme immobilization, role of nanomaterials, and also application of microfluidics for development of miniaturized enzymatic biosensors.
