ABSTRACT
Introduction Since its invention more than 400 years ago, light microscopy has become an indispensable tool for biological research (Kasper and Huang 2011). Among various light microscopy modalities, fluorescence microscopy (FM) is probably the most versatile and the most widely used (Lichtman and Conchello 2005). By labeling target molecules with fluorophores-molecules that can be excited by light in one wavelength and then emit light in another wavelength-FM can provide 3D images of a wide range of biomolecules in the context of cells up to whole living animals.
