chapter  16
In Vitro Calli Induction, Biomass Accumulation and Different Biological Activity of Leucas aspera (willd.) Linn.
ByMasilamani Sri Devi, Krishnamoorthy Vinothini, Blassan P. George, Sudharshan Sekar, Heidi Abrahamse, Bettine van Vuuren, Arjun Pandian
Pages 20

Leucas aspera (willd.) Linn, belongs to Laminaceae family and is a branched herbaceous plant, which is herb erected, stout and has a hispid acutely quadrangular stem and branches. The plant is used traditionally as an insecticide and antipyretic, the leaves are used for psoriasis, chronic skin eruptions, snakebites, gastrointestinal disorders and respiratory tract disorders. The seeds and in vitro calli were shade-dried and powdered; the powdered samples were cold macerated with different solvents for 3d with occasional stirring. Different concentrations of the leaf, stem and in vitro calli were loaded into the wells using a sterile micropipette. The antimicrobial activity by the zone of inhibition was observed and carried out for wild leaves, stems and in vitro calli in different solvents and extracts against the human pathogens B. subtilis, S. aureus, E. coli, S. typhi, V. cholera and C. krusei. The chapter concludes that work that the explants of L. aspera showed profuse in vitro biomass accumulation and root formation.