NMR of Subcellular Organelles — A Bioenergetic Viewpoint
The developments in nuclear magnetic resonance (NMR) spectroscopy have opened new avenues for both applied and basic medical research. The energy state and metabolic activities of cells and tissues are closely regulated phenomena. Under basal metabolic conditions, the activity of cellular ATPase(s), particularly in muscle, is lower than that in stimulated states, such as in contraction. The molecular nature of the coupling of electron transport to the formation of ATP has been, and continues to be, one of the major questions in modern biochemistry. According to the chemical hypothesis, electron transport causes the formation of high energy chemical intermediates which in turn transfer their energy to ATP. One of the most obvious solutions for obtaining the highest possible S/N ratio is to increase the length of observation (number of transients) at the expense of time resolution, aside from the customary NMR manipulations.