ABSTRACT
Karyologic analysis readily distinguishes dipteran cells from lepidopteran cells. Lepidopteran cells contain both macro- and microchromosomes, and the ploidy is quite variable. Isoenzyme analysis, employing the enzyme phosphoglucoisomerase (PGI) and isoelectric focusing (IEF), was successfully used for both intragenic and intergenic identification of lepidopteran cell lines. Intragenic and intergenic differentiation of lepidopteran cell lines from genera was made using IEF and PGI. The technique of IEF and isoenzyme analysis is well suited for the identification of insect cell lines. Identical isoenzyme patterns of H. zea and H. virescens cells with their homologous hemolymph were observed. Also, T. ni and S. frugiperda cell lines gave identical patterns with their hemolymph. The isoenzyme patterns of larval hemolymph and ovarian tissue for each of the species of Heliothis were identical. Only patterns from H. armigera and S. ornithogalli cell lines were different from their species or origin.
