ABSTRACT
This chapter considers some separations of a kind similar to argentation chromatography, but performed with salts of metals other than silver. If Ligand exchange chromatography (LEC) can be interpreted broadly enough to include all kinds of chromatography in which metal ions are held in the stationary phase and metal-ligand forces affect the retention of ligands, then argentation chromatography can be classified under LEC. The fact that ions of silver form complexes with olefins was used to advantage in chromatography by B. W. Bradford' in 1955. Argentation chromatography was applied to liquid chromatography. Argentation chromatography, particularly argentation-TLC, has gained wide acceptance and popularity for lipid research and analysis. Argentation adsorption chromatography of lipids was simultaneously reported by deVries and L. J. Morris. Olefins were retarded by silver nitrate and separated from saturated hydrocarbons. The olefin octadecene was held more strongly and was eluted, the Trans isomer, by pentene containing 1 to 3% ether.
