ABSTRACT
II. The Nature of the Adsorbent 3 A. Muscle: The Role of Actin and the I-Band 3 B. The Role of Tropomyosin (TM) and Troponin (TN) 4 C. The Specificity of Enzyme Binding Sites 5 D. Piggy-Back or Indirect Binding and Glycolytic Enzyme Organization.... 10 E. Nonmuscle Cells and Cytoskeletal Actin I I
III. Dynamics of Enzyme Organization 15
A. Metabolic Dependence of Enzyme Binding 15
B. Effectors of Binding 18
C. Genetic Determinants of Binding 20
IV. Functional Significance of Binding 20
A. Influence on Catalytic Expression 20
B. Structural Considerations 24
References 26
I. HISTORICAL PERSPECTIVE
While the classification of the glycolytic enzymes as soluble is an operational definition which reflects the relative ease with which these proteins are extracted from cells and tissues, the term also has the conceptual connotation that these enzymes exist free in solution within the cell. Unfortunately this implied intracellular distribution is still widely held despite the wealth of accumulated evidence that these enzymes are represented in both the particulate and soluble phases of the cell.
