ABSTRACT
Cytopathic effect was marked in the mammalian cells, but in reptile cells it was absent or minimal and subtle. Chaco virus grew to high titer in one of four reptile cell lines and in baby hamster kidney (BHK)-21 cells. Marco, Chaco, and Timbo virus particles exhibit all of the structural details of typical rhabdoviruses, but differences have been noted between Marco and the other two viruses in shape and size. The shape of Chaco and Timbo virus particles is cylindrical. Mean particle length is 202 nm, and budding as been seen only upon endoplasmic reticulum. Chaco and Timbo viruses resemble other rhabdoviruses with particle lengths longer than the prototype vesicular stomatitis virus. Timbo viruses have been cross tested against 30 other rhabdoviruses isolated from athropods and vertebrates, with negative results. Between 1955 and 1963, 4766 lizards of at least 14 species were examined for viruses at the Belem Virus Laboratory by intracerebral inoculation of organ suspensions into suckling mice.
