ABSTRACT
Embryo culture is frequently mistaken for a relatively simple procedure. In reality, it is a complex task, requiring proactive quality control and quality assurance programs to ensure the optimum performance of the laboratory and equipment, together with a high level of training for embryologists. Furthermore, a sufficient number of suitable incubation chambers are required to maintain a stable environment for development in vitro. Hence, embryo culture is far more involved than simply using the appropriate culture media formulations. Consequently, in order to optimize embryo development in vitro and maintain viability to ensure the delivery of a healthy baby, it is essential to consider embryo culture as a system in its entirety. The embryo culture system consists of the media, macromolecules, gas phase, type of medium overlay, the culture vessel, the incubation chamber, ambient air quality, and even the embryologists themselves. The concept of an embryo culture system successfully highlights the interactions that exist not only between the embryo and its physical surroundings, but also between all parameters within the laboratory (Figure 16.1). Only by taking such a holistic approach can one optimize embryo development in vitro and maintain success rates.
