ABSTRACT
Abstract ................................................................................................... 76 4.1 Introduction .................................................................................... 76 4.2 Material and Methods .................................................................... 79 4.3 Experimental .................................................................................. 80 4.4 Results and Discussion .................................................................. 84 4.5 Concluding Remarks ...................................................................... 88 Acknowledgment .................................................................................... 89 Keywords ................................................................................................ 90 References ............................................................................................... 90
KATARÍNA VALACHOVÁ1,*, DOMINIKA TOPOL’SKÁ1, MILAN NAGY2, IVO JURÁNEK1, and LADISLAV ŠOLTÉS1
1Institute of Experimental Pharmacology and Toxicology, SK 84104 Bratislava, Slovakia
2Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University in Bratislava, SK 83232 Bratislava, Slovakia
*Corresponding author. E-mail: Katarina.Valachova@savba.sk
ABSTRACT
The antioxidative activity of aqueous and methanolic extracts of Ayurvedic plants, Azadirachta indica A. Juss, Ocimum tenuiflorum L., and Withania somnifera (L.) Dunal were assessed by four in vitro methods. The hydrogen atom-donating property of the aqueous extracts of plants was investigated in accordance with their influence on the free radical-mediated degradation of high-molar-mass hyaluronan. Kinetics measurements to assess the electron donor capacity of the aqueous extracts were performed by the ABTS assay. Inhibitory activity of the aqueous extracts was assessed by means of site-specific hydroxyl radical-mediated 2-deoxy-d-ribose degradation (SRD) and standard spectrocolorimetric ABTS assay. The spectrocolorimetric DPPH assay was exploited to accomplish the determination of the IC50 values of methanol extracts of the three Ayurvedic plants.
