ABSTRACT
This chapter presents the research study on the synthesis of silver nanoparticle using human cells. The synthesized silver nanoparticles have potential application in drug discovery and drug delivery/targeting. Several other studies are in progress to find the location inside the cell, where the synthesis is occurring and to characterize the intra- and extracellular silver nanoparticles. This method could be a novel process of synthesis of silver nanoparticles using cell culture and will be applicable in the areas of drug targeting and delivery to the cancer cell. The cell lines utilized in the study were: human embryonic kidney cell, HeLa cell line and SiHa cell line. Transmission electron microscopy images show the additional data of the presence of silver nanoparticles in the cell lines. The cells attached to the wells were detached by trypsinization and were subsequently lysed following experimental protocol for cell lysis.
