ABSTRACT

Thin layer chromatography (TLC) and especially high performance thin layer chromatography (HPTLC) are flexible, fast, and inexpensive off-line separation techniques, suitable especially in phytoanalysis [1]. HPTLC, in comparison with TLC, allows a better separation owing to a smaller particle size of the stationary phase. Sample application for separation is done directly on the stationary phase and subsequently a solvent flows through the stationary phase achieved by capillary force

forming the mobile and the stationary phase. Special staining detection methods make TLC or HPTLC the method of choice for rapid analysis of medicinal plants and their preparations [2,3].