ABSTRACT

The starch-processing industry is the second largest segment of the industrial enzyme market, representing approximately 30% of world enzyme production. The major enzymes are amylases, glucoamylases,

7.1 Introduction ........................................................................................... 129 7.2 Microbial amylase producers .............................................................. 130 7.3 Industrial needs .................................................................................... 132 7.4 Approaches to enzyme improvement ................................................ 135

7.4.1 Isolation of new microbial strains .......................................... 135 7.4.2 Gene cloning and protein engineering .................................. 137

7.5 Applications of enzymatic modied starches .................................. 140 7.6 Final remarks ..........................................................................................141 References ........................................................................................................ 142

and glucose isomerases which are used for starch liquefaction, sacchari- cation, and isomerization.